鄂报春(Primula obconica Hance)亲缘地理学的初步研究

Phylogeography of Primula obconica Hance(Primulaceae)

为了获得鄂报春(PrimulaobconicaHance)的种内亲缘地理学信息,对叶绿体基因组上非编码区trnL-trnF的序列变异进行了分析。获得的20个单倍型具有一定的地理分布结构。对这些单倍型进行系统发育分析,得到了三个主要分支:东部分支广泛分布于中国东南部、湖南、湖北、四川和云南;四川分支分布于四川西部;云南分支分布于云南西北部。东部分支分布面积较后两个分支大,并且在东部分支中可以发现一定的演化关系。结合古气候学和地质学信息,推测东部分支中现存的单倍型由冰期时气候变冷导致鄂报春快速迁移而形成,云南、四川分支中的单倍型可能是在各自的避难所中得以保存。同时本文也简要地探讨了鄂报春几个亚种之间的分类学关系。

Chloroplast DNA trnL-F region was sequenced to investigate intraspecific phylogeography in Primula obconica Hance in China. Twenty haplotypes were recognized in total, and most of the haplotypes were geographically structured. There are three major clades in the most parsimonious trees obtained from those haplotypes. Eastern clade was widely distributed from Nanping, Fujian （eastern China） to Weixi, Yunnan （western China）. Sichuan clade was confined in western Sichuan, and Yunnan clade existed in north-western Yunnan. It was shown that the topology within Eastern clade was hierarchical slightly, and most of the haplotypes in Eastern clade mainly confined in Wuling Mountains and Nanling Mountains. The haplotypes in eastern clade might be correlated with the climate oscillation in the glacial period, which might migrate from western China to eastern China. In contrast, the haplotypes from Sichuan clade and Yunnan clade might survive in glacial period, suggesting there were several glacial refuges in western China. Taxonomical relationships among subspecies ofP. obconica were also discussed.