摘要
目的明确广州地区临床分离的耐药绿脓假单胞菌各种β内酰胺酶编码基因、外膜通道蛋白oprD2基因、氨基糖苷类修饰酶基因存在状况。方法采用MicroScan微生物鉴定系统微量肉汤法测定临床分离的18株绿脓假单胞菌对12种抗菌药物的敏感性,采用聚合酶链反应及序列分析的方法分析β内酰胺酶基因型、氨基糖苷类修饰酶基因型及外膜通道蛋白oprD2基因。结果该18株菌呈现多重耐药,对氨基糖苷类抗生素的耐药率在50.0%~72.2%之间,对其他抗绿脓假单胞菌药物耐药率在16.7%~83.3%之间;14株(77.8%)检出氨基糖苷类修饰酶基因,aac(6')Ⅱ、aac(3)Ⅱ、ant(3")Ⅰ、aac(6')Ⅰ、ant(2")Ⅰ、aac(3)Ⅰ、aac(3)Ⅲ、aph(3')Ⅵ和aac(3)Ⅳ基因的阳性率分别为50.0%、38.9%、38.9%、33.3%、27.8%、11.1%、5.6%、5.6%和0;13株(72.2%)检出β内酰胺酶编码基因,TEM、DHA、IMP和OXA基因阳性率分别为55.6%、27.8%、22.2%和11.1%,SHV、PER、VER、GES和VIM基因阴性,3株外膜通道蛋白oprD2基因缺失。结论广州地区临床分离的绿脓假单胞菌多重耐药严重,氨基糖苷类修饰酶基因和β内酰胺酶编码基因携带率很高。
Objective To investigate the genotyping of β-lactamases (BLs) and aminoglycosidemodifying enzymes(AMEs), and the distribution of the outer membrane protein OprD2 gene produced by multiresistant Pseudomonas aeruginosa(PA) isolated from Guangzhou. Methods Antimicrobial sensitivities of 18 strains of P. aeruginosa to 12 kind of antibiotics were determined by Bade MicroScan Wakaway-96. BLs AMEs OprD2 gene 18 trains of Pa were by polymerase chain reaction (PCR) and analyzed by DNA sequencing. Results The antibiotic resistance of PA was multiple. Most were resistant to aminoglycosides (50. 0% -72. 2% ) , and resistant rates to other antibiotics were 16. 7% to 83. 3%. 14 strains(77. 8% ) carried one or more types of AMEs gene , including aac (6') - Ⅱ ( 9 trains) , aac ( 3 ) - Ⅱ ( 7 trains ) , ant (3")- Ⅰ (7 trains) , aac(63- Ⅰ (6 trains) , ant(2" )-Ⅰ(5 trains) , aac(3)- Ⅰ (2 trains) , aac(3)-Ⅲ ( 1 train) and aph (3') -Ⅵ ( 1 train) . 13 strains (72. 2 % ) carried types of BLs gene, including blaTEM ( 10 trains) , blaD.A(5 trains) , blaCMP(4 trains) and blaOXA(2 trains) . None of the 18 strains of PA possessed genes of blas.v ,blapER, blaVER, blaGES, blaVIM and aac(3)-Ⅳ, and 3 isolates of PA lost the outer membrane protein OprD2 gene. Condutions The study showed that it is more serious issuer for multiresistant Pseudomonas aeruginosa carrying genes of BLs and AMEs in Guangzhou.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2005年第11期1211-1214,共4页
Chinese Journal of Laboratory Medicine
基金
CLONGEN细菌耐药基因研究专项基金资助项目(20040401HD)