绿脓假单胞菌β内酰胺类耐药基因研究

Study on resistant genes of beta-lactam antibiotics from clinical isolates of Pseudomonas aeruginosa

目的调查湖北襄樊地区绿脓假单胞菌多重耐药菌株中β内酰胺酶编码基因及oprD2基因存在状况。方法采用PCR方法检测分离自本地区的35株绿脓假单胞菌耐药菌株各种β内酰胺酶编码基因TEM、SHV、OXA、PER、GES、IMP、VIM、质粒型AmpC酶DHA、MIR及OprD2。结果35株β内酰胺类耐药基因TEM、OXA、质粒型AmpC酶DHA的阳性率分别为51·4%、17·1%、2·9%,OprD2基因缺失率100%,而SHV、PER、GES、IMP、VIM、MIR基因检测均阴性。结论研究表明,携带TEM、OXA、质粒型AmpC酶DHA以及OprD2基因缺失是导致本组绿脓假单胞菌多重耐药菌株对β内酰胺酶类抗生素耐药的重要机制。

Objective To investigate β-Lactamase coding genes and OprD2 gene in multidrug- resistant Pseudomonas aeruginosa isolated from Xiangfan region in Hubei province. Method Polymerase chain reaction（PCR） was used to detect various β-Lactamase coding genes including TEM,SHV,OXA, PER,GES,IMP,VIM,plasmid type AmpC β-Lactamase DHA ,MIR and OprD2 in 35 strains of Pseudomonas aeruginosa. Results The detection rate of β-Lactamase coding genes TEM, OXA, plasmid type AmpC β- Lactamase DHA were 51.4% ,17. 1% and 2. 9% respectively, all of the tested strains of Pseudomonas aeruginosa lossed OprD2 gene.but SHV,PER,GES,IMP,VIM,MIR genes were negative. Conclusion The study indicated that these Pseudomonas aeruginosa carried genes of TEM, OXA, plasmid type AmpC β- Lactamase DHA and lossed OprD2 gene. which was the essential resistance mechanism of Pseudomonas aeruginosa to Beta-lactam antibiotics in local aera.