江苏省宜兴地区丙型肝炎病毒基因分型研究

Study on hepatitis C virus genotyping in Yixing area, Jiangsu province

目的了解江苏省宜兴地区丙型肝炎病毒(HCV)基因型分布特征和病毒变异情况。方法对宜兴市人民医院收治的158例HCV抗体阳性患者血清进行HCV RNA检测,对阳性标本进行Simmonds分型,采用5′非编码区(5′NCR)1、2、3、1b型特异性引物进行PCR扩增,并对1b型和2 型各1株的PCR产物测序验证。分析不同性别、临床类型的丙型肝炎患者基因型分布差异。结果158份血清中,有95份为HCV RNA阳性,其中1b型80份(84.2%),2型5份(5.3%),1b/2型的混合感染5份(5.3%),不能分型的5份(5.3%),1b型的序列与GenBank J-4株的同源性为99.2%,2型与GenBank J-6株的同源性为97.7%。男性和女性在基因型总体分布上存在差异(P<0.05),并且在单一型感染和混合型感染的分布上差异亦有统计学意义,不同临床类型的丙型肝炎中HCV基因型分布未显示差异。结论江苏省宜兴地区HCV以1b型为主,男女患者HCV基因型分布差异有统计学意义,而不同临床类型的HCV基因型分布无显著差异,基本反映了本地区HCV感染的特点。

Objective To investigate the distribution of hepatitis C virus （HCV） genotypes in Yixing,Jiangsu province. Methods Genotypes identification on sera samples were obtained from 158 donors who had already been anti-HCV positive through PCR method with type specific primer designed according to the sequence of 5＇non-coding region （5＇NCR）. 5＇NCR was also sequenced and compared with published date. Genotypes distribution was investigated in patients with different sex and clinical types of hepatitis C. Results Of the total 158 patients, 95 were HCV RNA positive in which 80 patients having genotype 1b （80/95; 84.4 % ）, 5 patients having genotype 2（5/95;5.3 % ）, 5 patients with 1 b/2 mixed genotypes （5/ 95;5.3 %） and another 5 patients whose genotype undetermined. The difference on the distribution of HCV genotypes was significant between female and male patients （P〈 0.05） but not in different kinds of hepatitis C patients. Conclusion Type lb was the predominant HCV genotype in Yixing area.