HPLC测定皮湿霜中苯佐卡因、地塞米松磷酸钠和醋酸氯己定含量

HPLC Detemination of Benzocaine,Dexamethasone Sodium Phosphate and Chlorhexidine Acetate in Pishi Cream

目的:建立 HPLC 测定皮湿霜中苯佐卡因、地塞米松磷酸钠和醋酸氯己定含量的方法。方法:色谱柱:YWG C_(18)分析色谱柱(4.6mm×250 mm,10μm);流动相:甲醇-水(62:37,含0.3%三乙胺,磷酸调 pH 3.0);流速1 mL·min^(-1);检测波长240 nm。结果:苯佐卡因、地塞米松磷酸钠和醋酸氯己定的理论板数分别为5600,3500,3200;回归方程分别为:Y=1.382×10~7+1.550×10~7X(r=0.9998),Y=6.563×10~4+3.220×10~7X(r=0.9995),Y=-2.032×10~6+6.168×10~7X(r=0.9999);线性范围分别为6～18,0.09～0.27,0.6～1.8μg;平均回收率分别为97.6%(RSD=2.3%),96.8%(RSD=3.3%),102.4%(RSD=2.1%)。结论:本法操作简便,结果准确可靠,可用于皮湿霜中苯佐卡因、地塞米松磷酸钠和醋酸氯己定的含量测定。

Objective： A quantitative method was developed for the determination of benzocaine, dexamethasone sodium phosphate and chlorhexidine acetate in Pishi cream by using HPLC. Methods：The chromatographic conditions include column adopted YWG C 18 （4.6 mm × 250 mm, 10 μm ）, mobile phase ： methanol - water （ 62： 37, containing 0. 3% triethylamine solution）,adjusted to pH 3.0 with phosphoric acid,flow rate 1 mL· min6-1 ,detected wavelength 240 nm. Results： Theoretical plates counts for benzocaine, dexamethasone sodium phosphate and chlorhexidine acetate peaks were 5600,3500 and 3200 respectively, The regression equation for benzocaine, dexamethasone sodium phosphate, chlorhexidine acetate were Y = 1. 382 × 10^7 ＋ 1. 550 × 10^7 X （ r = 0. 9998 ）, Y = 6. 563 × 10^4 ＋ 3.220 × 107X（ r = 0. 9995 ）, Y = - 2. 032 × 10^6 ＋ 6. 168 × 1067X（ r = 0. 9999 ） ; and the corresponding linear ranges were 6 - 18 μg,0. 09 -0.27 μg,0. 6 - 1.8 μg,respectively. The average recoveries of the respective measurements were 97.6% （ RSD = 2.3% ） ,96. 8% （ RSD = 3.3% ） and 102. 4% （ RSD = 2. 1% ）, respectively. Conclusion： The method is sensitive, rapid and accurate.