AgNORs图像分析在睾丸畸胎瘤鉴别中的应用

DIFFERENTIAION OF THE BENIGN AND MALIGNANT TESTICULAR TERATOMAS BY USING THE IMAGE ANALYSIS OF AgNORs.

我们应用银染和图像分析技术，对睾丸正常组织、畸胎瘤、畸胎癌细胞进行了研究，结果发现：正常睾丸及畸胎瘤的ＡｓＮＯＲｓ颗粒呈圆形、规则、边界清楚、大小均匀，位于胞核偏中或边缘部位；畸胎癌的颗粒呈圆形或卵圆形、欠规则、边界欠清、大小较不一致，偏中或散在分布于核内。畸胎癌的ＡｓＮＯＲｓ数目、面积、大颗粒数、平均面积、圆度比正常及畸胎瘤的大（Ｐ＜０．０１），前三项参数在畸胎瘤比正常组大（Ｐ＜０．０１）。故我们认为ＡｇＮＯＲｓ图像分析对睾丸良恶性畸胎瘤的鉴别是有意义的，可为临床提供一项较为客观的指标。

he value of image analysis of silver-stained nucleolar organize(AgNORs)has been in-vestigated in normal testicular tissues,benign teratomas and malignant teratomas. The ma-jor findings were in normal tissues and benign teratomas the AgNORs are round and regtular.with clear boundaries and even sizes and localed in the center or at the margin of the nucleus;while in malignant teratomas, they are irregular with unclear boundaries and uneven sizesand scatter in the nucleus or gather in clusters. The number,area,large particle number, mean area and roundness of AgNORs weregreater in malignant teratomas than in normal tissties and benign teratomas(P<0.01).thenumber,area and large particle number were greater in benign teratomas than in normal tis-sues(P<(0.01). Ourresults showed that the image analysis of AgNORs was helpful in differentiating thebenign and malignant testicular teratomas and can provide the valuable marker for clinicaluse.