不同浓度NK4对多种肿瘤细胞侵袭能力的影响

Effect of NK4 in different concentrations on invasion of multiple tumor cells

目的研究不同浓度NK4因子对多种肿瘤细胞侵袭能力的影响。方法采用基底膜侵袭实验观察不同浓度NK4因子对人结肠癌、乳腺癌等多种肿瘤细胞侵袭能力的影响。结果在浓度为10μg/ml时,NK4因子对人结肠癌细胞、乳腺癌细胞、小鼠黑色素瘤细胞和人卵巢癌细胞侵袭重组基底膜的抑制率为56.4%,56.3%,56.1%和45.0%。结论NK4因子具有抑制人结肠癌、乳腺癌等多种肿瘤细胞侵袭的能力。

Objective To study the effect of NK4 （a four-kringle fragment of hepatocyte growth-factor） at different concentrations on invasion of multiple tumor cells. Methods The invasive capacity of human colon cancer and breast cancer cells was evaluated by reconstituted basement membrane invasion assay. Results At a concentration of 10 μg/ml, NK4 factor suppressed human colon cancer cells, breast cancer cells, mouse melanoma cells and human oophoroma cells that invaded the reconstituted basement membrane by 56.4%, 56.3%, 56.1% and 45.0%. Conclusion NK4 factor can inhibit the invasion of multiple tumor cells, such as human colon cancer cells and breast cancer cells.