阿霉素、5-Fu、三尖杉酯碱及去炎松对体外培养的人视网膜色素上皮细胞的抑制

Inhibition Effects of Doxorubicin, 5-Fluorouracil, Harringtonine and Triacinrolone Acetonide on Human Retinal Pigment Epithelial Cells in Vitro

应用3H-胸腺嘧啶核苷（3H－TdR）掺入及液体闪烁测量技术，观察不同浓度的阿霉素，5-氟尿嘧啶（5－Fu）、三尖杉酯碱及去炎松对体外培养的人视网膜色素上皮细胞（RPE）增殖的抑制作用。结果；阿霉素在2～32ng／ml时，对RPE细胞抑制率为85～77.2％，ID50为12ng／ml；5－Fu在0.2～3．2μg／ml时，对RPE细胞抑制率为14．5～81．7％，ID50为0．6μg／ml；三尖杉酯碱在2．5～40ng／ml时，抑制率为18．5～94．1％，ID50为4.5ng／ml；去炎松在200～350μg／ml时对RPE细胞抑制率为37．9～53．5％，ID50为340μg／ml。结论：阿霉素、5－Fu、三尖杉酯碱及去炎松能有效地抑制RPE细胞的增殖。

This study was to select drugs against cellular proliferation in the vitreous. Doxorubicin (2ng/ml-32ng/ml), fluorouracil (5-Fu) (0. 2ug/ml-3. 2ug/ml), harringtonine (2. 5ng/ml-40ng/ml ) and triacinrolone acetonide(TA) (150ug/ml-350ug/ml) were added to cultures of human retinal pigment epithelial(RPE) cells respectively and proliferation rates of the cells were measured with 3H-thymidine incorporation and liquid scintillation techniques on days. The results showed that doxorubicin, 5-Fu, harringtonine and TA inhibited cellular proliferation effectively and in a dose-dependent manner (doxorubicin, 8. 7-77. 5% ;5-Fu, 14. 5-81. 7%;harringtonine, 18. 5-94. 1%; TA, 18-53. 5% P<0.05). Their ID were 12ng/ml 0. 68ugg/ml, 4. 5ng/ml, and340ug/ml, respectively. Doxorubicin, 5-Fu,harringtonine and TA had an effective inhibition on human RPEcellular proliferation.