巨噬细胞刺激人成纤维细胞增生及去炎松和道诺霉素对其的抑制

Stimulation of Cultured Human Fibroblasts by Macrophages and Its Inhibition by Triamcinolone and Daunomycin

采用离体培养的人皮肤成纤维细胞并加入巨噬细胞调理培养液（MCM），及3H-胸腺嘧啶掺入液体闪烁检测，观察了成纤维细胞受MCM刺激后的增殖及药物对其的抑制作用。结果，加入MCM组细胞增殖率较对照组明显增加（P＜0．01），150ng／ml道诺霉素及联合用药组的细胞抑制率最高达95％以上（P＜0．01），250μg／ml去炎松的抑制率在不加MCM组约为20％～40％，而在加MCM组约为40％～60％（P＜0．01）。证实去炎松除有直接抑制作用外，更能明显抑制巨噬细胞活性因子对成纤维细胞增生的刺激作用。

Human skin fibroblasts cultured with macrophage-conditioned medium(MCM) and/or drugs wereobserved with phase microscopy and liquid scintillography. The proliferation of fibroblasts in the culture withMCM increased significantly when compared to that of controls (P<0. 01). Daunomycin (150ng/ml) alone orcombined with triamcinolone acetonide (250ug/ml) inhibited the cell growth up to 95% (P<0.01). Triamicinolone produced inhibition of approximately 20% to 40% in the culture without MCM, and 40% to 60% inthe culture with MCM (P<0.01). The results indicate that triamicinolone has more effects of eliminating thestimulation to fibroblast proliferation by macrophage-derived factors in addition to limited direct inhibition ofthe cell growth.