摘要
应用鼠伤寒沙门菌株ＴＡ９８及其新的衍生菌株ＹＧ１０２１和ＹＧ１０２４检测了液化石油气（ＬＰＧ）燃烧产物接触者尿的致突变性。吉果表明，在有大鼠Ｓ_９已β－葡萄糖醛酸酶存在时，尿样显示较强的致突变性。尿样对ＹＧ１０２４的致突变性明显高于ＴＡ９８，而对ＹＧ１０２１的致突变性与ＴＡ９８相近。提示ＬＰＧ燃烧产物接触者尿中致突变物主要是以葡萄糖醛酸贰结合状态存在的芳香胺类物质。同时也证明ＹＧ１０２４在检测该尿样的致突变性上比ＴＡ９８更为敏感。
The mutagenicity of urine from humans exposed to the combustion products of liquefied petroleum gas(LPG)was detected with Salmonella typhimurium TA98 and its new derivatives YG1021 and YG1024. The samples showed evident mutagenicity in thepresence of both rat liver S_9 and β-glucuronidase.The mutagenicity in YG1024 was significantly higher than that in TA98. The mutagenicity in YG1021,however,was similar to TA98 under the same conditions. This suggests that the mutagenic compounds presented in the urine samples are mainly aromatic amino compounds as glucuronide conjugates.The results also demonstrate that YG1024 is more sensitive than TA98 in detecting the mutagenicity of these samples.
基金
国家自然科学基金
关键词
液化石油气
燃烧产物
尿致突变性
liquefied petroleum gas
combustion product
urine mutagenicity
Ames test
