摘要
研究了雷公藤内酯酮对小鼠变应性接触性皮大、Ｔ和Ｂ淋巴细胞增殖反应及脾细胞分泌白细胞介素２（ＩＬ－２）活性的影响。发现雷公藤内酯酮１、２、５ｍｇ／ｋｇ腹腔注射（ｉｐ，ｑｄ×５ｄ）能显著抑制二硝基氟苯（ＤＮＦＢ）诱导的变应性接触性皮炎，而对脾细胞ＩＬ－２分泌活性无显著影响。体外试验表明，０．０２、０．１０、０．５０ｍｇ／Ｌ浓度的雷公藤内酯酮能显著抑制刀豆素Ａ（ＣｏｎＡ）及脂多糖（ＬＰＳ）诱导的Ｔ、Ｂ淋巴细胞增殖反应。
Triptonide (Tri)extracted from Tripterygium wilfordii Hook inhibited the proliferation of mouse splenocytes induced by suboptimal concentration of concanavalin A or Lipoplysaccharide at concentrations of 0.02, 0.10,and 0.50 mg/L.Although allergic contact dermatitis to dinitrofluorobenzene (DNFB) was inhibited by Tri 1.0, 2.0,and 5.0 mg/kg(ip,qd×5d),the splenocyte interleukin-2 secretion activity of these mouse was not suppressed.The data indicates that Tri has suppressive effects on mouse immune functions and may be used as a potent immunosppressive agents.
关键词
雷公藤内酯酮
接触性皮炎
triptonide
allergic contact dermatitis
lymphocytes
interleukin-2
mice
