摘要
采用Zorbax Eclipse XDB-C8(4.6mm内径×150mm,5μm)色谱柱,乙腈流动相,流速0.70mL/min,检测波长407nm,建立了测定标题化合物的反相高效液相色谱法.该方法的线性范围是0.5～12.0μg/mL,回归方程为A=82.06c+10.16(r=0.9996),相对标准偏差3.218%(c=6.0μg/mL,n=5),检出限为0.05427μg/mL,平均回收率在96.81%～105.1%之间.
A reversed phase high performance liquid chromatographic method for separation and determination of 1-（4-nitrobenzene）-3-（5-chloro-2-pyridyl）-triazene was described. Chromatography was carried out on Zorbax Eclipse XDB-C8 column with acetonitrile as mobile phase. The calibration curve was linear over the range from 0.5 - 12.0μg/mL（ r = 0.9996） .The RSD of the method was 3. 128% （c = 6.0μg/mL, n = 5 ）and minimum detectability 0.05427μg/mL. Recovery of the method was 96.8% - 105.1%.
出处
《化学试剂》
CAS
CSCD
北大核心
2005年第11期665-666,共2页
Chemical Reagents
