影响枯草芽孢杆菌fmbR菌株抗菌物质提取的主要因子

The main factors affecting the extraction of the antimicrobial substance from Bacillus subtilis fmbR strain using Plackett-Burman Design

应用P lackett-Burm an Design对枯草芽孢杆菌fmbR菌株抗菌物质提取的主要影响因子(甲醇、乙醇、丙醇、正丁醇、pH值和时间)进行了研究。JMP软件分析结果表明:影响抗菌物质提取的关键因素为乙醇(P<0.000 1)、正丁醇(P<0.000 1)、pH值(P=0.002 5)和时间(P=0.008 3)。在实验范围内,乙醇、正丁醇和时间对抗菌物质提取总量有显著正效应,pH值与抗菌物质提取总量呈显著负相关。抗菌物质提取总量最高预测值可达21.82 mg.100 mL-1,其概率为99.79%。

In order to evaluate the importance of the selected six factors, including methanol, ethanol, propanol, butanol, pH and time, on the extraction of the antimicrobial substance produced by Bacillus subtilis fmbR strain, the Plackett-Burman Design method was applied. The main factors that affect the extraction of the antimicrobial substance were determined as ethanol （P 〈0. 000 1 ）, butanol （ P 〈0. 000 1 ）, pH （ P =0. 002 5 ）, time （ P =0. 008 3 ） by using JMP software. Within the tested ranges, ethanol, butanol and time showed the significant positive relativity to the total extracted amounts; while pH had a significant negative effect. The maximum prediction profile indicated that the total extracted amounts for the antimicrobial substance would reach 21.82 mg·100 mL^-1 with 99.79% probability.