诱导免疫耐受控制垂体移植免疫排斥Ⅰ.去脑垂体大鼠免疫耐受性的诱导

Controling Immunologic rejection of Pituitary Transplantation By inducing Tolerance Ⅰ.Experimental Study of Inducing Donor-specific Tolerance In Hypophysectomized rat

在立体定各仪经耳去除交系ＳＤ大鼠脑垂体基础上，用不同剂量近交新生的Ｗｉｓｔａｒ大鼠制成活化脾细胞和环磷酰胺进行诱导免疫耐受性的研究。结果：活化脾细胞５×１０＾７／０．５ｍｌ／Ｗ或５×１０＆６／０．５ｍｌ／Ｗ联合环磷酰胺２０ｍｇ／ｋｇ／ｄ持续２周后，去脑垂体大鼠同种异体皮肤移植自下而上时间分别为ＭＳＴ〉３３．５天和ＭＳＴ〉４７．８天。

Inbred spraque-Dawly female rats were hypophysectomized through the transeardrum stereotactic approach.Donor spleen Cells(SC)were isolated from inbred newborn wistar rats and activated by ConA.We induced the donor-specfic tolerance with the following processes,i.e.(1) injectong several doses of activated Spleen cells(ASC)onto the recipent(Hpox-SD rats)intravenously. (2)Cyclophosphamide(CP)intraperitoneally.(3)Combining ASC and CP.The result showed that ASC or ASC plus CP can promote skin allograft survial(MST>33.5 days and MST>56 days).The above results lead us to think that ASC or ASC plus CP can successfully induce donor-specfic tolerance in hypophysectomy rats.