人工骺板软骨对家兔骺板损伤的修复作用

Repair of artificial epiphyseal plate cartilage on epiphyseal plate injury of rabbits

目的:探讨人工骺板软骨移植修复骺板损伤.方法:切除家兔左侧胫骨上端骺板软骨约1/3～1/2,制备人工骺板软骨构件移植入该骺板损伤处;右侧胫骨近端骺板损伤后不进行移植作为对照.连续观察家兔双下肢外观及运动状态,摄双侧下肢X线片,测量胫骨角进行比较.行HE染色、甲苯胺蓝染色及免疫组化进行组织形态学观察.结果:移植8周时双下肢胫骨角,对照组为(38.80±13.50)°,人工骺板软骨移植组为(9.01±4.2)°;移植24周时对照组为(54.25±18.33)°,人工骺板软骨移植组为(11.27±5.1)°,对照组与人工骺板软骨移植组相比差异均有显著性(P＜0.01).术后2周,对照组胫骨近端骺板内侧缺损处有骨桥形成,无软骨修复组织;而实验组移植后2周时,骺板缺损区已由软骨修复充填,移植的骺板软骨细胞排列未见层次及柱状排列状态,与正常骺板软骨相邻处分界明显;移植4周以后的骺板软骨细胞开始出现柱状排列状态,层次较明显.移植的骺板软骨Ⅱ型胶原染色阳性.结论:人工骺板软骨应能够修复骺板软骨损伤,效果满意.

Objective To investigate the possibility of repairing the epiphyseal plate injury using artificial epiphyseal plate. Methods The left tibial epiphyseal plate of rabbit was resected about 1/3 to 1/2, then the artificial epiphyseal plate were transplanted; while the right side was injured but not proceed transplantation as contrast. The tibial angle was measured after X-ray check. T test was used for data analyzing. HE stain, toluidine blue Dyeing and immunohistochemistry test were performed to observe the histomorphology. Results The tibial angle of lower extremity was （38.80±13.50）° in control group, （9. 01±4.2）° in artificial epiphyseal plate group 8 weeks after transplantation;（54.25±18. 33）° in control group, （11.27±5.1）° in artificial epiphyseal plate group 24 weeks after transplantation. There was significantly difference between control group and artificial epiphyseal plate group （P〈0. 01）. There was bony bridge formation in control group. 2 weeks after planting, the epiphyseal plate impairment region was filled with cartilage tissue, but the chondrocytes in the transplanted epiphyseal plate were changed into irregular arrangement, and demarcation was distinct with normal epiphyseal plate cartilage. After planting for 4 weeks, cartilage layers were noted in the treatment groups and the cutting edges disappeared. There were collagen-Ⅱ-positive cells in transplanted epiphyseal plate cartilage. Conclusion The artificial epiphyseal platecan repair the epiphyseal plate injury, and the effect is satisfied.