黄蘑多糖提取物的抗肝癌作用及其机制

Anti-liver cancer effect of Huangmo Polysaccharides extracts and its mechanism

目的:研究黄蘑多糖提取物的抗肝癌作用及其机制。方法:建立小鼠体内移植性肝癌模型,黄蘑多糖水溶性提取物(FI)腹腔连续给药,测定抑瘤率(IR);MTT法检测H22荷瘤小鼠淋巴细胞转化功能、NK细胞活性及IL-2、TNF-α的含量。结果:FI 20、40和80 mg.kg-1剂量组对肝癌H22的平均抑瘤率分别为36.1%、47.7%和57.6%,表现出较强的抗肿瘤活性;能增加体外免疫器官重量,促进T淋巴细胞转化,提高NK细胞及IL-2、TNF-α的活性。结论:FI为生物反应调节剂,通过增强机体免疫功能的作用达到抗肿瘤的功效。

Objective To research the effect of Huangmo Polysaccharides extracts on liver cancer and its mechanism. Methods The bearing tumor mouse model with Hepatoma-22 was established for anti-tumor experiment. Huangmo Polysaccharides extracts （FI） was administered, i. p, its inhibitory rate of tumor （IR） was dtected. The lymphocyte transformation, the activity of NK cells, and the contents of IL-2 and TNF-a were determined with MTT assay. Results After treated with FI of 20, 40, 80mg·kg^-1 , the inhibition rates （IR） on Hepatoma-22 were 36.1%, 47.7%, 57.6%, respectively; It had a good activity on anti-liver cancer. FI increased the weights of immune organs, improved the lymphocyte transformation and the activities of NK ceils, I1-2 and TNF-α. Conclusion As biological response modifier, FI has effects of anti-liver cancer through increasing the activity of body immunity, improving the lymphocyte transformation and the activities of NK cells, I1-2 and TNF-α.