葛根对中国仓鼠肺细胞的毒性作用

Toxic effect of puerarin on lung fibroblasts of Chinese hamsters

目的:观察葛根对中国仓鼠肺细胞(CHL)形态学、细胞增殖活性及细胞相对存活率的影响。方法:以葛根为受试物,采用噻唑蓝(MTT)比色法检测不同浓度的葛根在不同作用时间对CHL相对存活率的影响。结果:不同浓度的葛根(12.50、50.00、100.00、200.00和400.00 mg.L-1)作用一定时间后,CHL存活率下降,细胞增殖明显受到抑制并出现细胞脱落,呈现出时间-剂量效应关系。接触受试物48 h后,细胞生长半数抑制浓度(IC50)为282.00 mg.L-1。结论:葛根可以抑制CHL生长,降低线粒体代谢活性。

Objective To observe the effects of puerarin on the morphology, cell proliferation activity, and cell relative survival rate of Chinese hamster lung cells （CHL）. Methods MTT colorimetric assay was used to detect the effects of puerarin with different concentrations at different time on the survival rate of the CHL. Results The survival rates of CHL were obviously decreased under the exposure of various concentrations （12.50, 50.00, 100.00, 200.00, 400.00 mg· L^-1） of puerarin, proliferation of CHL was significantly inhibited and some cells detached from the wall in puerarin groups and presented a dose-time dependent manner, the 50% inhibited concentraion （IC50） for cell growth was 282 mg· L^-1. Conclusion Puerarin might inhibit the proliferation and the metabolic activity of mitochondria of CHL.