复发性口腔溃疡患者白细胞介素2及抑制性T细胞的检测

Detection of IL-2 and T suppressor cell in patients with recurrent oral ulcer

目的:探讨复发性口腔溃疡(ROU)的免疫发病机制。方法:应用CTLL法和短寿命抑制性T细胞(Ts)功能测定技术,对36例ROU患者和41例正常对照组外周血白细胞介素2(IL-2)活性和Ts功能进行检测,并观察转移因子治疗后IL-2及Ts的变化。结果:ROU患者外周血单个核细胞产生IL-2活性水平明显低于正常对照组(P<0.01),Ts抑制功能与正常对照组比较差异无显著性(P>0.05)。经转移因子治疗后ROU患者IL-2活性水平较治疗前明显提高(P<0.05),转移因子治疗前后ROU患者Ts功能变化差异无显著性(P>0.05)。结论:ROU患者细胞免疫功能低下及T细胞亚群功能失衡与本病发病有关;转移因子治疗ROU可提高ROU患者IL-2活性。

Objective To investigate the immunologic mechanism of recurrent oral ulcer （ROU）. Methods The IL-2 activities and T suppressor （Ts） cell functions in peripheral blood of 36 ROU patients were detected with CTLL and short-term Ts cell functional detection technique. Results The IL-2 activity of single-nuclei cells in peripheral blood of ROU patients was lower than that in normal controls （P〈0. 01）, while the suppressing function of Ts cells had no difference between the two groups. The IL-2 activity of ROU patients increased sifgnificantly after treated with transfer factor compared with before treatment （P〈0. 05）, while the Ts cell function did not change after treatment （P 〉 0. 05）. Conclusion The ROU patients have a low cell immune function and an imbalanced T cell sub-clone, which may be related to the development of ROU. The effect of transfer factor on ROU is closely related to its function of up-reguating IL-2 activity.