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人肝细胞癌抗原HCA661 DNA疫苗的构建 被引量：1

Construction of DNA vaccine of human hepatocellular carcinoma antigen HCA661
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摘要 目的:构建人肝细胞癌特异性抗原HCA661的DNA疫苗。方法:通过PCR从SMMC7721中获得编码肝细胞癌特异抗原HCA661的基因组DNA序列,插入pGEM-T easy载体后测序,并将目的基因定向亚克隆进入真核表达载体pCI-neo,以PCR和酶切方法鉴定构建的重组真核表达质粒pCI-neo-HCA661。结果:PCR产物为1 040 bp,测序结果与Gene Bank登记完全相同;PCR和酶切结果表明,构建的重组真核表达质粒中含有正确编码的目的基因片段。结论:成功地构建了含有HCA661基因的DNA疫苗。
作者 李树蕾 谭岩 刘力华 段秀梅 方艳秋 姜艳芳 许淑芬 王忠山
机构地区 吉林大学基础医学院组织胚胎学教研室 吉林大学第一医院中心实验室 吉林大学基础医学院细胞生物学教研室
出处 《吉林大学学报（医学版）》 CAS CSCD 北大核心 2005年第6期957-959,共3页 Journal of Jilin University：Medicine Edition
基金 吉林省科技厅重点项目资助课题(20040401-5) 吉林省科技厅国际合作项目资助课题(20040707-2) 长春市科技局计划项目资助课题(2004218) 高等学校博士学科点专项科研基金资助课题(20020183017)
关键词 HCA661基因 癌/睾丸抗原 疫苗 DNA
分类号 Q782 [生物学—分子生物学] Q785 [生物学—分子生物学]
  • 相关文献

参考文献11

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共引文献47

同被引文献10

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引证文献1

二级引证文献1

吉林大学学报（医学版）
2005年 第6期

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