盐酸小檗硷对人肝Bel-7402细胞株LDLR表达的影响

Effects of berberine on the expression of LDLR in human liver cell strain Bel-7402

目的:探讨盐酸小檗硷(BBR)在体外对人肝Bel-7402细胞株LDLR表达的影响。方法:采用不同浓度的BBR与人肝Bel-7402细胞株共同培养不同的时间后,通过RT-PCR、流式细胞术及Western-Blotting技术分别检测LDLRmRNA、LDLR及过氧化物酶体增生物激活受体γ(PPAR-γ)的表达。结果:经BBR处理过的肝细胞的LDLRmRNA表达明显增加,且其表达强度与所加入的BBR浓度及及作用时间呈正相关;最小有效剂量是0.25μg/ml,最短起效时间是2h,24h仍保持较高的表达水平。而PPAR-γ的mRNA表达与对照组相比无显著性差异(P>0.05)。结论:BBR能通过增加LDLR的表达而有效调节细胞对脂蛋白的摄取和代谢,从而维持细胞外LDL-C水平的稳定。

Objective ： To investigate the effects of berberine （BBR） on the expression of low-density lipoprotein receptor （LDLR） of human liver cell strain Bel-7402. Methods： LDLR and PPAR-γ mRNA and protein expression levels were detected by RT-PCR, flow cytometry and Western-Blotting after the co-incubation of BBR with different concentrations and human liver cell strain Bel-7402 for various time-spans. Results： The levels of LDLR mRNA after the co-incubation were significantly higher than those of controls（P 〈 0.05 or P 〈 0.01）,and were closely related to the BBR concentration and the incubating time. The shortest effective time of coincubation was 2 h, and the high level could last for 24 h. However, the expression of PPAR-γ was not changed after incubation（P 〉 0.05）. Conclusion： BBR can effectively regulate the absorption and metabolism of human liver cell strain Bel-7402, and maintain the stabilization of the LDL-C level outside the cell.