脑卒中患者血管紧张素转换酶基因缺失多态性分析

Analysis of ACE gene deletion polymorphism in patients with cerebrovascular diseases

目的:研究血管紧张素转换酶(ACE)基因多态性与脑卒中之间的相关性。方法:应用PCR技术,对98例脑血管疾病患者(分为脑血栓形成组、腔梗组和脑出血组)和52例对照组人群分别检测其基因频率和基因型频率。结果:D∶I等位基因比率脑卒中组为0.61∶0.39,对照组两者之比为0.43∶0.57,两组比较具有统计学差异(P<0.05),DD型基因两组相比差异也具有统计学意义(43/98vs10/52,P<0.05)。有高血压的脑卒中患者DD型基因及D等位基因频率较对照组增加(P<0.05),有、无脑卒中家族史患者DD型基因及D等位基因频率无明显改变(P>0.05)。结论:ACE基因I/D多态性是脑卒中特别是脑血栓及腔梗重要的危险因素,高血压病史与脑卒中患者ACEI/D多态性相关,而脑卒中家族史与之无关。

Objective： To discuss the relationship between the ACE gene deletion polymorphism and cerebrovascular diseases. Methods： We detect the frequency of the deletion allele D and the polymorphism DD by using PCR techniques in 98 patients with cerebrovascular diseases compared with 52 normal age and sex method controls. Results： The allele frequency was 0.61 ： 0.49 in patients with cerebrovascular diseases and 0.43：0.57 in controls （P 〈 0.05）. The DD genotype was more common in patients with cerebrovascular diseases compared with controls （43/98 vs 10/52, P 〈 0.05）. The incidences of polymorphism DD and the frequency of the deletion allele D were higher in cerebral stroke with history of high blood pressure （P 〈 0.05）. While the incidence of the polymorphism DD and the frequency of the deletion allele D had no differences in cerebral stroke with or with：out family history of cerebral stroke （P 〉 0.05）. Conclusion ：Our study shows the deletion polymorphism in the ACE gene may be a risk factor for both lacunar stroke and cerebral embolism. The deletion polymorohism in the ACE gene is associated with high blood pressure.