肠胃清药物血清对人口腔表皮癌细胞株KB-A-1多药耐药的逆转作用

Reversal of mulitdrug resistance by Changweiqing oral liquid on human oral epidermoid carcinoma cell line KB-A-1

目的评价中药复方肠胃清口服液对肿瘤细胞多药耐药的逆转作用。方法采用连续灌胃给药3d后的SD大鼠的肠胃清药物血清,作用人口腔表皮癌敏感细胞株KB-3-1和耐药细胞株KB-A-1,运用MTT等方法,观察肠胃清对KB-3-1和KB-A-1细胞的生长抑制作用及对KB-A-1细胞的多药耐药的逆转作用。结果肠胃清药物血清在2．5％～20％浓度时对KB-3-1和KB-A-1细胞的生长均有抑制作用,呈剂量依赖性,对KB-A-1和KB-3-1的IG_(50)分别是(8．5±1．7)％和(10±1．6)％。在对KB-3-1和KB-A-1细胞的生长无毒性的含药血清浓度为2．5％、5％的作用下,阿霉素对KB-A-1细胞的IC_(50)从(10．3±0．9)μg／mL分别下降到(4．2±0．2)μg／mL和(2．0±0．1)μg／mL,逆转倍数分别达到了2．5倍和5．2倍,而对KB-3-1细胞则不产生逆转作用。结论肠胃清可有效逆转多药耐药细胞KB-A-1的耐药作用。

Objective To explore the effect of Changweiqing oral liquid（CWQ） on reversal of multidrug resistance on cancer cells. Methods Observe the effect of CWQ drug serum, which was obtained from SD rats that had been intragastred for 3 d, for growth inhibition in KB-3-1 cell line and KB-A-1 cell line and reversal of muhidrug resistance by MTT assay. KB-A-1 cell line was drug resistance one, KB-3-1 cell line was sensitive one. Results CWQ could inhibit the cell-growth of KB-3-1 and KB-A-1 cell line with dosage dependence, when the contents of drug serum were 2.5%-20%. The ICs0 of KA-A-1 and KA-3-1 were （8.5 ± 1.7） % and （10 ± 1.6）%, respectively. When the content drug serum without cytotoxicty for KB-3-1 and KB-A-1 were 2.5% and 5 %, the IC50 of KB-A-1 in doxorubicin deceased from （10.3 ± 0.9）μg/mL to （4.2 ± 0.2）μg/mL and （2.0 ± 0.1） μg/mL, the reverse muhiple reached 2.5 and 5.2 ,respectively, however, the serum could not reverse drug resistance in KB-3-1 cell line. Conclusion CWQ significantly reverses mulitidrug resietance in KB-A-1 cell line.