白藜芦醇抗喉癌作用的研究

Research of a new kind of biological response modifier to anti-laryngocarcinoma

目的:通过对白藜芦醇(Res)抗喉癌作用的研究,筛选一种新型的抗喉癌生物反应调节剂。方法:采用四氮唑溴盐(MTT)法测定经Res处理后的Hep-2细胞的生长抑制率,并以流式细胞术分析细胞周期,检测细胞凋亡;建立喉癌小鼠模型,检测NK细胞杀伤率、刺激指数、抗体释放量及IL-2I、L-8、TGF-β1和VEGF的含量。结果:Res呈时间-剂量依赖性抑制Hep-2细胞增殖,促进细胞凋亡,出现明显的凋亡峰和G0/G1期阻滞现象;荷瘤小鼠生长状况改善,免疫功能增强。结论:Res作为一种生物反应调节剂,能提高机体抗肿瘤免疫应答水平,调节细胞因子分泌,发挥抗喉癌作用。

Objective： To find a new kind of biological response modifier to anti-laryngocarcinoma through studying the efficiency of resveratrol. Method：To detect the growth inhibiting rate of Hep-2 exposed to resveratrol with MTT colorimetry and to analyze the apoptosis and cell cycle of Hep 2 with flow cytometry. As having formed the model of laryngocarcinoma, quite a few indicatrixes including the content changes of IL 2, IL-8,TGF-β1 and VEGF were detected. Result： Resveratrol could induce apoptosis of Hep-2 in time-concentration-dependent manners showing obvious cell cycle blocking of G0/G1 and apoptotic peak. Furthermore, resveratrol improved remarkablely the growth condition of mice planted with Hep-2 while enhancing their immunological function. Conclusion： As a kind of biological response modifier, resveratrol could strengthen anti tumor immunoresponse, accommodating lymphocyte to secrete cytokin, through which it exerted the efficiency of anti-laryngocarcinoma.