摘要
目的探讨子宫内膜癌组织中雌激素受体(ER)亚型mRNA及孕激素受体(PR)亚型mRNA和蛋白表达水平的变化及其意义。方法采用RT-PCR法检测66例子宫内膜癌和30例正常子宫内膜组织ER、PR亚型mRNA的表达,采用蛋白印迹法检测PR亚型蛋白的表达。结果(1)ERαmRNA在子宫内膜癌和正常子宫内膜组织中的表达水平分别是8.00±7.77、3.84±2.57,而ERβmRNA的表达水平分别是4.15±3.55、0.41±0.29,子宫内膜癌组织中ERα、ERβmRNA表达水平均高于正常子宫内膜,两种组织间分别比较,差异均有统计学意义(P<0.05)。(2)PR、PRA和PRB蛋白表达及PR mRNA表达的降低与子宫内膜癌的发生有关(P<0.05),而PRA与PRB蛋白表达的比值和PRB mRNA的表达与子宫内膜癌的发生无明显相关性(P值分别为0.550、0.901)。(3)子宫内膜癌组织中PRB mRNA与ERβmRNA的表达水平呈正相关关系(r=0.43,P<0.01)。结论子宫内膜癌组织中ER亚型mRNA表达上调、PR蛋白和mRNA表达的下调可能参与了子宫内膜癌的发生;PRB mRNA与ERβmRNA表达呈正相关关系。
Objective To investigate the expressions of estrogen receptor （ER） and progesterone receptor （PR） subtypes in human endometrial carcinoma tissues. Methods Thirty samples of normal endometrial tissue and 66 endometrial carcinomas were investigated using western blot assays and reversetranscription polymerase chain reaction. Results （1）Both ERα and ERβ mRNA were significantly increased in endometrial carcinoma compared with that in normal endometrium tissues. The relative value of ERα and ERβ mRNA in endometrial carcinoma was 8.00 ± 7.77, 3.84 ± 2. 57 and that in normal endometrium tissues was 4. 15 ±3.55, 0. 41 ±0. 29, respectively. （2） Decreased levels of PR, PRA, PRB protein and PR mRNA were associated with endometrium carcinogenesis （ P 〈 0. 05 ）, while PRB mRNA expression and the ratio of PRA/PRB was not. （3） Expression of PRB mRNA was associated with ERβ mRNA （r = 0. 43, P 〈 0. 01 ）. Conclusion Overexpression of ER subtypes and down-regulation of PR may be involved in the pathogenesis of endometrial carcinoma, and expression of PRB mRNA is associated with ERβ but not ERα mRNA.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2005年第11期752-755,共4页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金资助项目(30170977)
关键词
受体
雌激素
受体
孕酮
子宫内膜肿瘤
Receptors, estrogen
Receptors, progesterone
Endometrial neoplasms
