CO_2对结肠癌细胞表面黏附分子mRNA表达的影响

Effect of persistent CO_2-insufflation on mRNA expression of adhesion molecules of colon cancer cell lines

目的探讨不同压强持续性CO2压力对结肠癌细胞表面黏附分子mRNA表达的影响。方法建立体外气腹模型,选用人结肠癌细胞株SW1116,分别在6、9、12和15mmHg压强下,以99%医用CO2气体以及常规培养条件下暴露1h,采用实时荧光定量PCR检测细胞表面黏附分子0,12,24,48和72h的表达变化。结果SW1116细胞株E-cadherin,ICAM-1,CD44,CD44v6mRNA在处理后表达增高,与处理前差异有统计学意义,在处理后的72h之前表达降至或低于处理前水平。E-cadherin、CD44v6和ICAM-1随压强增高其表达量逐渐降低,在处理后0h各压强组差异有统计学意义。结论持续性CO2压力对结肠癌细胞表面的黏附分子mRNA的表达产生一过性双向影响;随CO气体压强增高,黏附分子的表达遭到抑制。

Objective To investigate the effect of CO2-insufflation on mRNA expression of adhesion molecules of colorectal cancer cell lines. Methods With an artificial pneumoperitoneum model in vitro, SWII16 human colon carcinoma cell lines were exposed to CO2-insufflation of different pressure （6 mm Hg, 9 mm Hg, 12 mm Hg and 15 mm Hg） for 1 hour. The mRNA level of E-cadherin, ICAM-1 , CD44 and CD44v6 were measured after 0, 12, 24, 48 and 72 hours by real-time RT-PCR. Control cell lines were exposed to room air. Results The mRNA expression of E-cadherin, ICAM-1, CD44 and CD44v6 increased significantly at 0 h -48 h after the cells were exposed to the continuous CO2-insufflation for 1 hour （P 〈 0. 05 ）, and returned to or were even less than the control levels by 72 h（P 〈 0. 05 ）. With the increase of the CO2-insufflation pressure, the expression of the E-cadherin, CD44v6 and ICAM-1 decreased （P 〈 0.05 ）. Conclusions The expression of adhesion molecules of cancer cells experiences a biphasic alteration under continuous CO2-insufflation and is negatively correlated with CO2 pressure.