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利用激光显微切割技术提取高质量RNA 被引量：3

Application of the microdissection distill the high quality RNA in the tumor research
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作者 陈凌 付丽
机构地区 天津医科大学附属肿瘤医院乳腺病理研究室
出处 《中华病理学杂志》 CAS CSCD 北大核心 2005年第10期680-682,共3页 Chinese Journal of Pathology
基金 国家自然科学基金资助项目(30470667) 教育部留学回国人员研究经费(2004-2005)
关键词 RNA 显微切割技术 质量 提取 培养的肿瘤细胞 肿瘤组织 激光 间质细胞 实验研 研究对象
分类号 R361 [医药卫生—病理学]
  • 相关文献

参考文献19

  • 1Emmert-Buck MR, Bonner RF, Smith PD, et al. Laser capture microdissetion. Science,1996,274:998-1001.
  • 2Burgemeister R, Gangnus R, Haar B, et al. High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology. Pathol Res Pract,2003,199:431-436.
  • 3Qin Y, Heine VM, Karst H, et al. Gene expression patterns in rat dentate granule cells:comparison between fresh and fixed tissue. J Neurosci Methods,2003, 131:205-211.
  • 4Van Deerlin VM, Gill LH, Nelson PT. Optimizing gene expression analysis in archival brain tissue. Neurochem Res, 2002,27:993-1003.
  • 5Specht K, Richter T, Müller U, et al. Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue. Am J Pathol, 2001,158:419-429.
  • 6胡盛平,张可浩,沈忠英,吴名耀,邓小玲,杨捷生,方福德.手工组织显微切割食管癌不同病变阶段细胞提取高质量RNA[J].中国医学科学院学报,2003,25(6):659-663. 被引量：6
  • 7Goldsworthy SM, Stockton PS, Trempus CS, et al. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol Carcinog, 1999,25:86-91.
  • 8韩为农,李虹,王爽,周文,姚开泰.显微切割结合RNA线性扩增技术的应用[J].癌症,2002,21(9):1022-1025. 被引量：7
  • 9江培洲,沈新明,黄华,石益民,姚开泰.显微操作仪快速分离癌细胞并提取微量RNA的方法[J].第一军医大学学报,2002,22(6):551-553. 被引量：3
  • 10Lindeman N, Waltregny D, Signoretti S, et al. Gene transcript quantitation by real-time RT-PCR in cells selected by immunohistochemistry-laser capture microdissection. Diagn Mol Pathol, 2002,11:187-192.

二级参考文献14

  • 1J萨姆布鲁克 EF弗里奇 T曼尼阿蒂斯.现代生物技术译丛:分子克隆实验指南：第2版[M].北京:科学出版社,1999.343.
  • 2[1]http://mecko.nichd.nih.gov/lcm/lcm.htm
  • 3[2]http://www.olympuscojp/LineUP/Microscope/biological/indexEhtml
  • 4[3]http://derisilab.ucsf. edu/pd fs/RNA Amplification.pdf
  • 5[4]http://www.bio.davidson.edu/courses/Molbio/MolStudents/spring 2000/kizer/catkizer SMART.html
  • 6[5]Datson NA, van-der-Perk-de-Jong J, van-den-Berg MP. et al. Micro SAGE: a modified procedure for serial analysis of gene expression in limited amounts of tissue [J ]. Nucleic Acids Res, 1999, 27 (5):1300-7
  • 7[1]Moskaluk CA, Kern SE. Microdi- ssection and polymerase chain reaction amplification of genomic DNA from histological tissue sections [J]. Am J Pathol, 1997,150(5):1547- 1552.
  • 8[2]Eberwine J. Amplification of mRNA populations using aRNA generated from immobilized oligo(dT)- T7 primed cDNA [J]. Biotechniques, 1996, 20(4):584- 591.
  • 9[3]Walch A, Specht K, Smida J, et al. Tissue microdissection techniques in quantitative genome and gene expression analyses [J]. Histochem Cell Biol,2001,115(4):269- 276.
  • 10[4]Brady G, Iscove NN. Construction of cDNA libraries from single cells [J]. Methods Enzymol,1993,225:611- 623.

共引文献11

同被引文献20

  • 1石善溶,石砚,Clive R.Taylor.抗原修复技术研究进展[J].中华病理学杂志,2007,36(1):7-10. 被引量：14
  • 2骆新兰,许洁,刘艳辉,庄恒国.HE染色切片褪色后免疫组织化学染色抗原修复方法[J].中华病理学杂志,2007,36(1):59-60. 被引量：5
  • 3郗彦凤,白文启,王晋芬,王全红,焦士兰.B细胞特异性激活蛋白/CD30双标记在经典型Hodgkin淋巴瘤中的表达及意义[J].中华病理学杂志,2007,36(2):136-137. 被引量：2
  • 4Moon WS, Park HS, Yu KH, et al. Expression of betacellulin and epidermal growth factor receptor in hepatocellular carcinoma : implications for angiogenesis[J]. Human Pathology, 2006, 37(10) : 1324-1332.
  • 5Rogers,B. B.Overview of non-Hodgkin’s lymphoma. Seminars in Oncology Nursing . 2006
  • 6Armitage JO,Weisenburger DD.New approach to classifying non-Hodgkin‘s lymphomas: clinical features of the major histologic subtypes. Non-Hodgkin‘s Lymphoma Classification Project. Journal of Clinical Oncology . 1998
  • 7Catalano,A.,Iland,H.Molecular biology of lymphoma in the Microarray era. The Journal of Pathology . 2005
  • 8Ishii M,Hashimoto S,Tsutsumi S et al.Direct comparison of GeneChip and SAGE on the quantitative accuracy in transcript profiling analysis. Genomics . 2000
  • 9Yang G P,Ross D T,Kuang W W,et al.Combining SSH and cDNA microarrays for rapid identification of differentially expressed genes. Nucleic Acids Research . 1999
  • 10Rubin EM,Tall A.Perspectives for vascular genomics. Nature . 2000

引证文献3

二级引证文献3

中华病理学杂志
2005年 第10期

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