富含亮氨酸的间质蛋白多糖在小鼠牙周组织发育中的组织学定位

Immunohistochemical localization of leucine-rich proteoglycans in the developing periodontal tissues of mice

目的研究富含亮氨酸的蛋白多糖类物质纤维调节素(FMN)、核心蛋白聚糖(DCN)及双糖链蛋白聚糖(BGN)在小鼠牙周组织不同发育时期的分布特点,探讨其在牙周组织发育中的作用。方法取出生后第5、10、15、25天的BALB/c小鼠36只,引颈处死,解剖并分离含下颌第一磨牙区的下颌骨,新鲜配制4%多聚甲醛固定24h,甲酸-甲酸钠复合脱钙液脱钙1～3周,常规石蜡包埋,近远中向5μm连续切片。免疫组织化学Power VisionTM二步法,观察各发育时期第一磨牙牙周组织中3种间质蛋白多糖的组织学分布特点。结果FMN在牙龈结缔组织和牙周膜中呈强阳性表达,且在牙周膜与牙骨质及牙槽骨界面有较强表达;DCN强阳性表达于牙龈结缔组织、靠近牙槽骨面的牙周膜及牙槽骨表面的成骨细胞中;BGN则在各期牙龈结缔组织及牙周膜中表达,在牙槽骨表面及成骨细胞中为阴性。结论FMN可能与DCN及BGN相互作用,调节牙龈结缔组织及牙周膜胶原纤维的网络形成,并可能参与牙槽骨和牙骨质的矿化过程。

Objective To study the distribution and expression of fibromodulin, decorin and biglycan in developing normal periodontal tissues, so as to understand its role in periodontal tissue formation. Methods Thirty six BALB/c mice in different developing stages were killed and their bilateral mandibular first molars with surrounding alveolar bones and gingival tissues were taken out, Power VisionTM two steps immunohistochemical method with anti-fibromodulin, anti-decorin and anti-biglycan was used to detect the tissue distribution and cellular localization of fibromodulin and related proteoglycans, decorin and biglycan. Results Fibromodulin was strongly expressed in the subcutaneous gingival connective tissue, periodontal ligament, mainly in gingival and periodontal fibroblasts as well as their matrices. Strong expression was also noted in the area close to the interfaces of periodontal ligament-alveolar bone and periodontal ligamentcementum. Decorin was strongly expressed in the area of gingival connective tissue, periodontal ligament and the surface of alveolar bone, while biglycan was stained evidently in gingival connective tissue throughout the period of investigation, but negative in the surface of alveolar bone and osteoblasts. Conclusions Fibromodulin may interact with decorin and biglyean to regulate the network formation of gingival connective tissues and periodontal collagen fibers, and may be involved in mineralization of the alveolar bone and cementum.