Wnt信号传导通路组成蛋白与口腔鳞状细胞癌的分化和增殖

Roles of Wnt-1, β-catenin and adenomatous polyposis coli in the differentiation and proliferation of oral squamous cell carcinoma

目的研究信号传导通路组成蛋白Wnt-1、β-连环蛋白(β-catenin)和腺瘤样结肠息肉病(adenomatous polyposis coli,APC)基因在口腔鳞状细胞癌中的表达,探讨它们在口腔鳞状细胞癌分化和增殖中的作用。方法免疫组织化学法检测66例不同分化程度的口腔鳞状细胞癌中Wnt-1、β-连环蛋白、APC基因及MIB-1的表达。结果37例高分化口腔鳞状细胞癌中分别有30、25和31例高表达Wnt-1、APC和β-连环蛋白,7、12和6例低表达Wnt-1、APC和β-连环蛋白;29例中、低度分化口腔鳞状细胞癌中分别有6、9和11例高表达Wnt-1、APC和β-连环蛋白,23、20和18例低表达Wnt-1、APC和β-连环蛋白;66例口腔鳞状细胞癌中MIB-1有34例低表达,32例高表达。Wnt-1、β-连环蛋白和APC的表达与MIB-1和口腔鳞状细胞癌的组织学分级差异有统计学意义(P<0.01)。结论Wnt-1、APC和β-连环蛋白在口腔鳞状细胞癌的分化和增殖中起重要作用。

Objective To investigate the expressions of Wnt-1, β-catenin and adenomatous polyposis coli （APC） in oral squamous cell carcinoma （OSCC）. Methods Surgical specimens from 66 OSCC patients were examined for Wnt-1, β-catenin, APC and MIB-1 expressions by immunohistochemical staining. Results Among all the 37 cases of well differentiated OSCCs, there were 30,25 and 31 cases of high expressions of Wnt-1 ,APC and β-catenin, respectively, 7,12 and 6 cases of low expressions. Among all the 29 cases of moderate and poor differentiated OSCCs , there were 6,9 and 11 cases of high expressions of Wnt-1 ,APC and β-catenin respectively,23,20 and 18 cases of low expressions. Among all the 66 cases of OSCCs , there were 32 cases of high expressions of MIB-1 and 34 cases of low expressions. Expressions of Wnt-1, β-catenin and APC showed significant difference in different differentiation of OSCC. Conclusions Wnt-1,β-catenin and APC expressions were related to the differentiation of OSCC.