六种粘接剂的牙本质粘接界面纳米渗漏比较

Comparison of dentin nanoleakage among six adhesives

目的检测6种粘接剂粘接处理后牙本质粘接界面的纳米渗漏情况。方法选取12颗无龋人磨牙,用600目碳化硅砂纸在流水冲洗下预备出统一的牙本质粘接面玷污层。分别用6种粘接剂按使用说明进行粘接处理,切割成0.9mm厚试件,避光贮存于氨化硝酸银溶液中24h。透射电子显微镜下观察牙本质粘接界面的纳米渗漏情况,单因素方差分析比较各组银染颗粒分布面积比的均值。结果6种粘接剂的牙本质粘接界面在电子显微镜下均观察到明显的纳米渗漏,其中材料A[邦多,(16.09±2.08)%]、材料B[Single Bond,(13.39±1.81)%]和材料C[Prime&Bond NT,(11.27±1.94)%]之间的差异均有统计学意义(P<0.05);材料D[B1自酸蚀底涂剂,(12.13±2.11)%]与材料E[ClearfilTMSE Bond,(12.35±2.60)%]的差异无统计学意义,但两者的纳米渗漏均小于材料F[AdperTMPromptTM,(14.93±2.67)%],差异有统计学意义(P<0.05)。结论6种牙科粘接剂的牙本质粘接界面均存在不同程度的纳米渗漏。

Objective To evaluate the nanoleakage appearance in 6 dentin adhesives. Methods Superficial occlusal dentin of 12 human molars without caries extracted freshly was exposed with 600-grit SiC papers under running water. Six adhesives were applied to dentin surfaces according to the manufacturers＇ instructions. The bonded teeth were sectioned occluso-gingivally into 0.9-mm-thick serial slabs that were stored in ammoniacal silver nitrate solution in the dark for 24 h and prepared for transmission electron microscope. The extent of nanoleakage among 6 dentin adhesives was measured and compared using one-way ANOVA. Results The nanoleakage was observed obviously at the dentin-resin interfaces of all 6 adhesives. The average dentin nanoleakage values of Group A [BangDuo, （ 16. 09±2. 08 ） % ], Group B [ SingleBond, （ 13.39±1.81 ） % ], and Group C [ Prime＆Bond NT, （ 11.27±1.94 ） % ] were all significantly different （P 〈0. 05）. There was no significant difference in the dentin nanoleakage between Group D[ B1 , （12. 13 ±2. 11 ） % ] and Group E [ ClearfilTM SE Bond, （ 12.35±2. 60） % ], but both of them were lower than Group F [ AdperTM PromptTM, （14. 93±2. 67）%].Conclusions The nanoleakage phenomena at the dentin-resin interface were all observed diversely in the contemporary dental adhesives.