摘要
本研究用脱氧鸟苷培养处理鸡趾屈腱，去除其抗原呈递细胞后做活体异体移植．通过混合淋巴细胞培养实验、术趾主动屈曲功能、移植腱腱周粘连定量、羟脯氨酸含量、生物力学测定、血管染色、大体形态及组织学形态观察等证实处理腱免疫源性显著降低，活体异体移植后达到自体腱移植相同良好效果，而对照未处理新鲜异体移植腱被排斥．并发现增生活跃的纤维细胞生成细胶原原纤维（直径３３．３ｎｍ），形成以细胶原原纤维为主并与原粗胶原原纤维（直径３１７．０ｎｍ）并存的新生移植腱．
Flexordigitorumiumprofundustendonofthechickenswereculturedinthepresenceofdeoxyguanosine(dGua)todepletethecelularantigenofthetendonscelswithoutafectingtheirsur-vival.Theyweregraftedintothemajorhistoincompatiblerecipients.Thefreshhomologousandtheau-togeneictendonsweretransplantedforthecontrolobservations.TheresultsofthemixedlymphocyteculturesuggestedthattheimunogenicityofdGua-treatedtendonswassignificantlydecreased.Examina-tionofthedigitalactiveflexionfunctionindicatedthattheextentofadhesion,thehydroxyprolinelevels,thebiomechanicsanalysis,themacroscopicandmicroscopicandtheultrastructuralobservationsofthegraftsdemonstratedthatthedGua-treatedhomograftsweresuccessfulastheautograftsandtheuntreat-edfreshalograftsfailed.Thenewlyformedtendonswerecomposedofalargeamountoffinecolagenfibrils(diameter33.3nm)andfewthickcolagenfibrils(diameter317.0nm),whiletheuntreatedhomo-graftswererejectedandultimatelytransformedtolooseconnectivetissue.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1996年第3期175-178,共4页
Chinese Journal of Orthopaedics
