胸腺内移植脾细胞延长大鼠移植肝存活的研究

Prolongation of Hepatic Allograft Survival in Rats Receiving Intrathymic Splenocyte Transplantation

探讨大鼠胸腺内移植供体脾细胞在建立特异性供体不反应性中的作用。TJR/1大鼠作供体,SD大鼠为受体。切取供体鼠脾脏制成睥细胞悬液,注入到受体鼠胸腺内,此前两天受体鼠口服环孢素40mg·kg^(-1)·d^(-1),胸腺内脾细胞移植前皮下注射地塞米松25mg/kg。10天后取同一供体肝脏进行原位肝移植,术后不用免疫抑制剂。处理组半数以上大鼠存活超过100天,对照组存活9±1.51天。胸腺内移植了第3品系大鼠脾细胞的受体鼠,在接受了JR/1大鼠的移植肝后也短期死亡。表明这种供体不反应性是特异的。组织病理学及超微结构也证实脾细胞预处理的移植肝受损轻,排斥反应不明显。本研究提示了肝移植抗排斥反应的新途径。

This stduy was aimed to investigate the effects of intrathymically transplanted donor splenocytes on the establishment of donor-specific unresponsiveness to the hepatic allografts. Male TJR/1 and SD rats were used as liver donor and recipients respectively. Isolated splenocytes from TJR/1 rat spleen were inoculated into the thymus of 8 SD rats pretreated with oral cyclosporine 40 rng/kg daily for 2 days and given 2- 5mg/kg dexamethasone subcutaneously at inoculation. Ten days later the liver from the TJR/1 donor used to provide spleen cells was transplanted orthotopically to the SD rat that had received intrathymic splenocytes. Donor-specific unresponsiveness allowed 6 to 8 hepatic allografts to survive indefinitely without further immunosupression. Eight control SD rats treated without in-trathymical splenocyte transplantation rejected their hepatic allografts at 9+1. 15 days post operative-ly. All the rats in another control group intrathymically inoculated with third party spleen cells died at 8. 13 + 2. 23 days after liver transplantation. These results showed that the pretreatment with intrathymic splenocyte inoculation could induce donor-specific immunologic unresponsiveness to hepatic allograft and make it possible for the hepatic allograft to survive indefinitely without immunosup-pressants.