^(60)Co-γ辐照对坛紫菜原生质体诱变的研究

Mutagenesis on protoplast of Porphyra haitanensis by ^(60)Co-γ

利用60Co-γ射线对坛紫菜(Porphyrahaitanensis)原生质体进行辐照诱变,发现原生质体对60Co-γ射线有极强的耐受性。前期的培养结果表明,原生质体是一种理想的诱变材料。诱变结果表明,60Co-γ射线对坛紫菜可诱变剂量范围很大,从200~1300Gy均有突变株生成。诱变后细胞的存活率不宜作为坛紫菜诱变最佳剂量的指标。

^ 60Co-γ ray was used to induce mutant on protoplast of Porphyra haitanensis. The results showed that the protoplasts of P. haitanensis could survive under a wide range of γ-rays. Primary culture showed that the protoplast was suitable for mutagenesis. Further results also showed that mutants could be induced in a very wide range of dosages of ^60Co-γ ray. The mortality was not a suitable parameter for the evaluation of the mutant-induction efficiency in P haitanensis.