生防细菌BMY-1对几种植物病原真菌的抑制作用

Inhibitory effects of the biological control bacterium-BMY-1 on some plant pathogens

采用平板对峙培养法和滤纸片培养法测定细菌BMY-1菌株对番茄灰霉病菌(Botrytis cinerea)、辣椒疫霉病菌(Phytophthora capsici)、玉米小斑病菌(Bipolaria maydis)、玉米弯孢霉叶斑病菌(Curvularia lunata)等11种植物病原真菌生长的抑制作用。结果表明,BMY-1菌株对供试病原菌生长具有很好的抑制效果。平板对峙培养法中,BMY-1菌株对番茄灰霉病菌、玉米弯孢霉叶斑病菌、玉米小斑病菌的抑制效果最好,抑菌宽度分别达到15.0 mm,14.0 mm,13.0mm。滤纸片培养法中,BMY-1菌液对番茄灰霉病菌抑制效果最好,抑菌带宽度为8.0 mm。BMY-1菌液及其无菌发酵液对病原菌孢子萌发也有很强的抑制作用。其中对辣椒疫霉病菌和玉米小斑病菌孢子萌发的抑制效果最好。BMY-1菌液处理后,孢子萌发率分别为0.97%和1.00%,而对照萌发率为分别为96!29%和93.04%。

Inhibitory effects of the biological control bacterium-BMY-1 on 11 species of fungus pathogens, such as Botry- tis cinerea, Phytophthora capsici, Bipolaria maydis, Curvularia lunata, were measured by plate cultivation and filter paper cultivation. Results showed that BMY-1 had significant inhibitory effects. In plate relay experiments. In plate cultivation experiments, the inhibitory strips of Botrytis cinerea, Curvularia lunata, Bipolaria maydis were 15.0 mm, 14.0 mm, 13.0 mm respectively in breadth. In filter paper experiments, the inhibitory strips of Botrytis cinerea were 8.0 mm in breadth in filter paper cultivation experiments. BMY-1 solution and its sterile ferment liquid showed effective inhibition on the development of spore germination, effectiveness on Phytophthora capsici, Bipolaria maydis was better than on others. The spore germination rates of Phytophthora capsici, Bipolaria maydis were 0.97%, 1.00% respectively after treated by BMY-1 solutions, while that of CK was 96.29%, 93.04% respectively.