5-LOXmRNA和VEGFmRNA在胰腺癌中的表达及其临床意义

Expression of 5-LOXmRNA and VEGFmRNA in pancreatic cancer and its clinical significance

目的研究5脂氧合酶(5-LOX)在胰腺癌中的表达及其与血管内皮生长因子(VEGF)的关系。方法用RT-PCR检测5-LOX mRNA和VEGFmRNA在3 5例胰腺癌新鲜组织中的表达。结果5-LOX mRNA和VEGFmRNA在胰腺癌组织中的表达率分别为7 4.3%及6 0%,均与胰腺癌的临床分期有关;VEGF还与胰腺癌的分化程度有关。5-LOX mRNA和VEGF有协同作用(P<0.0 5),呈密切正相关(r(0.5 3 5,P<0.0 1)。III^IV期5-LOX mRNA表达明显高于I^II期(P<0.0 5)。结论5-LOX mRNA和VEGFmRNA在胰腺癌中表达增高,与临床分期有关。5-LOX mRNA和VEGF mRNA表达呈正相关。

Objective To investigate the expression of 5- LOX in pancreatic cancer tissue and the relationship between 5-LOX expression and expression of VEGF. Methods The expression of 5-LOX mRNA, VEGFmRNA in 35 pancreatic cancer fresh tissue samples were detected by semi-quantitive reverse transcriptase-polymerase chain reaction method. Results Expression of 5-LOX mRNA, VEGF mRNA in pancreatic cancer tissue were 74. 3% , 60% respectively, and the expression was correlated to the with clinical stages of the tumor; also expression of VEGF mRNA was correlated to the differentiation of the tumor. Expression of 5 - LOX mRNA and VEGF mRNA were synergetic in pancreatic cancer （ P 〈 0.05 ） , and were closely correlated （ P 〈 0.01 ） . Ⅲ - Ⅳ stages of pancreatic cancer showed significantly stronger expressions of 5-LOX compared to in Ⅰ - Ⅱ stages of pancreatic cancer （ P 〈 0. 05 ） . Conclusions Expression of 5-LOX, VEGF in .pancreatic cancer tissue was markedly elevated, and correlated to the clinical stages of the tumor; expression of 5-LOX and VEGF were positively related in pancreatic cancer.