不同产地壮药土垅大白蚁菌圃指纹图谱的研究

Studies on fingerprints of ant nest of Macrotermes annadalei from different areas

目的:对广西不同产地壮药土垅大白蚁菌圃指纹图谱进行比较研究.方法:采用全自动氨基酸分析仪测定12批不同产地土垅大白蚁菌圃的总氨基酸类成分.分离柱(4.6 mm×40mm),柱温57℃;缓冲液流速0.4mL·min-1,茚三酮流速0.3 mL·min-1.结果:12批样品的指纹图谱中共检出18个共有指纹峰,各样品重叠率为89.4%～100.0%,7批样品中8个主要峰的相对总面积均＞52%.结论:广西不同产地土垅大白蚁菌圃药材的氨基酸组分非常相似,大部分样品的品质较优.指纹图谱可为土垅大白蚁菌圃药材的鉴别和质量评价提供有用的信息.

Objective： To study comparatively the fingerprint of zhuang medicinal material-ant nest of Macrotermes annadalei from different areas in Guangxi. Methed： The fingerprint of total amino acid of 12 lots of ant nest of M. annadalei from different areas was determined by high speed amino acid analyzer. Separation was perform on anylytical column （4.6 mm × 40 ram）, column temperature at 57 ℃. The flow rate of buffeting solution was 0.4 mL. min^- l, and the flow rate of ninhydrin was 0.3 mL· min^-1. Result： 18 characteristic peaks in the fingerprints were identified in 12 lots of samples, the chromatographic overlap rate was 89.4%-100.0%, and the total relative peak area of 7 lots of samples was over 52% in eight main peaks.Conclusion： The components of amino acid of ant nest of M. armadalei from different areas in Guangxi are very similar. The qualities of majority samples are good as well. The fmgerprints can provide the useful information for the quality evaluation and the identification of ant nest of M. armadalei.