抗白念珠菌IgY对白念珠菌生长及黏附的影响

目的观察抗白念珠菌IgY对白念珠菌生长及体外黏附上皮细胞的影响。方法在含有不同浓度IgY的沙氏液体培养基中加入白念珠菌,37℃培养24h后,观察白念珠菌的生长现象;分离人口腔黏膜上皮细胞,用不同浓度IgY分别对上皮细胞和白念珠菌预处理后进行黏附抑制实验。结果加入IgY抗体后,白念珠菌的繁殖数量无明显变化,但生长过程中出现凝集现象,且随IgY浓度的升高而增加;口腔黏膜上皮细胞表面黏附的白念珠菌数目明显减少。结论IgY不影响白念珠菌的繁殖分裂,但可使其发生凝集,并能阻断其黏附上皮细胞,作用机制可能与IgY封闭白念珠菌的表面受体有关。

Objective ： To study the effect of anti-candida albicans IgY on the growth and adherence of candida albicans to epithelium in vitro. Methods： After cultured with IgY of different concentrations at 37C for 24h,the growth of candida, albicans was observed; The human buccal epithelium cells were isolated and produced adherence inhibition experiment after pretreating candida, albicans and buccal epithelium cells with different cdncentrations of IgY. Results： The IgY did not inhibit the growth of candida, albicans,but the agglutination was occurred; The IgY could significantly decreased the number of candida albicans adhering to the surface of buccal epithelium cells. Conclusion： Anti-candida albicans IgY can inhibit adherence of candida albicans to epithelium cells and the mechanism may be involved with IgY blocking surface receptor of candida albicans.