无花果曲霉（NRRL　3135）与黑曲霉（AsN　70）植酸酶产酶条件的研究

Study on Conditions of Producing Phytase by Asp. ficuum NRRL 3135 and Asp.niger 70

本文重点进行了无花果曲霉（Ａｓｐ．ｆｉｃｕｕｍＮＲＲＬ３１３５）和黑曲霉（Ａｓｐｎｉｇｅｒ７０）用固体培养法生产植酸酶的对比研究。结果表明：ＮＲＲＬ３１３５与ＡｓＮ７０均可以麸皮为主要原料，ＮＲＲＬ３１３５用缓冲液（ｐＨ４．８）控制ｐＨ，在３０℃±１℃培养８～９，产酶水平为５．２ｕ／ｇ干基；ＡｓＮ７０在不需要缓冲液控制ｐＨ的情况下，３０℃±１℃培养５～６天，其产酶水平可达４５．０ｕ／ｇ干基。

We have compared the conditions of producing phytase by Asp ficuum NRRL 3135 and Asp.niger 70 in solid state fermentation process.The results show that NRRL 3135 and ASN 70 all can use wheat bran as solid medium;and it would be beneficial for NRRL 3135 to have good pH control(with buffer),NRRL 3135 were incubated at 30±1℃ for 8～9 days,it can produce 5.2 u/g, but AsN 70 were incubated at 30±1℃ for5￣6 days,without buffer,it can produce 45.0u/g.