摘要
以丹参(SalviamiltiorrhizaBunge)无菌苗的茎、叶为外植体,接种于附加2,4-D、NAA、6-BA及其组合的MS固体培养基上。结果表明:单独使用三种植物生长调节物质在一定浓度范围内均能产生愈伤组织;配合使用外源生长素和细胞分裂素,能获得出愈率高、生长快、质量好的愈伤组织;最佳组合的诱导培养基为MS+2,4-D2.0mg/L+6-BA1.0mg/L;2,4-D对丹参愈伤组织的诱导有促进作用;但当2,4-D的浓度在4mg/L以上时,对愈伤组织的生长有抑制作用。
Salvia miltiorrhiza Bunge aseptic stems and leaves were used as explants cultured in solid medium MS supplemented with 2,4-D, NAA, 6-BA or combinations of plant growth regulators with different concentrations. The results showed that all the three regulators could induce callus under certain concentration ranges. Better results could be obtained by combining three regulators, the optimum combination was Ms+2,4-D2.0mg/L+6-BA1.0mg/L. Higher concentration of 2,4-D (over 4rag/L) restrain callus growth.
出处
《中国农学通报》
CSCD
2005年第11期202-204,275,共4页
Chinese Agricultural Science Bulletin
基金
陕西省十五科技攻关项目"主要药用植物的生长发育与有效成分积累研究"(2001KG01-G15-03)。
关键词
丹参
愈伤组织
生长调节物质
诱导
Salvia miltiorrhiza Bunge, Callus, Plant growth regulator, Callus induction