不同生长调节物质对丹参愈伤组织的诱导效应

A Study on the Effect of Different Growth Regulators on Callus Induction of Salvia miltiorrhiza Bunge

以丹参(SalviamiltiorrhizaBunge)无菌苗的茎、叶为外植体,接种于附加2,4-D、NAA、6-BA及其组合的MS固体培养基上。结果表明:单独使用三种植物生长调节物质在一定浓度范围内均能产生愈伤组织;配合使用外源生长素和细胞分裂素,能获得出愈率高、生长快、质量好的愈伤组织;最佳组合的诱导培养基为MS+2,4-D2．0mg／L+6-BA1．0mg／L;2,4-D对丹参愈伤组织的诱导有促进作用;但当2,4-D的浓度在4mg／L以上时,对愈伤组织的生长有抑制作用。

Salvia miltiorrhiza Bunge aseptic stems and leaves were used as explants cultured in solid medium MS supplemented with 2,4-D, NAA, 6-BA or combinations of plant growth regulators with different concentrations. The results showed that all the three regulators could induce callus under certain concentration ranges. Better results could be obtained by combining three regulators, the optimum combination was Ms＋2,4-D2.0mg/L＋6-BA1.0mg/L. Higher concentration of 2,4-D （over 4rag/L） restrain callus growth.