快速检测转移在同一张膜上的不同抗原

Determinate Rapidly of Different Antigen on the Same Transfer Membrane

用几种不同的抗体检测转移在同一张硝酸纤维素膜上的不同抗原,采用依次加入变性剂的再生法,变 性剂为1．5％SDS、150 mmolβ-巯基乙醇。再生温度:60℃,时间:30 min。结果:混合抗原中的几种目的 抗原均得以成功检测,且检测结果比独立检测法更为快捷方便,可作为同一张膜上的不同抗原的检测方法。

To determine the different antigen on the same transfer membrane, a method to regenerate membrane with denaturant was set up. The regeneration condition is 1.5%SDS, 150 mmol 2-mercaptoethanol, heating 30 min at 60 ℃. It is conclused that the method for determination of diffient antigen on the same transfer membrane with diffient antibody was rapid and effective.