空肠弯曲菌43kD优势抗原诱导抗DNA自身抗体类型分析

Isotype Analysis of Autoantibodies Against DNA Induced by 43kD Immunodominant Antigen of Campylobacter Jejuni

在发现空弯菌４３ＫＤ外膜通道蛋白不但是ＨＳＰ６０家族成员和优势抗原而且能诱导小鼠生产自身免疫应答的基础上，本文近一步分析了４３０ＫＤ优势抗原诱导产生针对ｄｓ－ＤＮＡ、ｓｓ－ＤＮＡ、ＥＮＡ、组蛋白４种自身抗体的类型和意义。结果表明１．被诱导的抗ｄｓ－ＤＮＡ、ｓｓ－ＤＮＡ抗体主要是１ｇＧ，其次才是ｌｇＭ型，偶见１ｇＡ型。２．被诱导的抗ＤＮＡ、组蛋白自身抗体主要是ＩｇＭ型和ＩｇＧ型。３．ＩｇＭ类抗ＤＮＡ自身抗体和ＩｇＧ类同时出现，这和前者产生细胞可能是后者产生细胞的前体这样一种推论矛盾。４．ＩｇＧ、ＩｇＭ抗ｄｓ－ＤＮＡ抗体高峰和阳性率导以抗９８－ＤＮＡ抗体，提示正常Ｂ细胞库中对ｓｓ－ＤＮＡ的免疫耐受更完全，识别ｄｓ－ＤＮＡ的Ｂ细胞克隆数量多和／或轻易被选择活化。５．本文表明４３ＫＤ优势抗原是空弯菌感染诱导自身免疫综合征小鼠模型中的关键性致病分子之一，为感染因子ＨＳＰ诱导自身免疫病的理论假设提供了一种直接的实验证据。

In these years,we have successfully established a muine model with systemic autoimmune syndromes induced by chronic infection or immunization of Campylobacter jejuni,a common pathogen being infectious both in human and animals,and have found that 43 KD protein(HSP43)of C.jejuni was not only the major component of outer membrane proteins and immunodominant antigen but also a member of HSP60 family.In this paper we studied isotype of autoantibodies induced by by 43kDa immunodominant antigen of C jejun and got some results:1.isotype of autoantibodies against ds-DNA and ss-DNA was mainly IgG and then IgM;2.isotype of autoantibodies against ENA and histones was mainly IgM and then IgG;3 IgM and IgG against DNA were induced simultaneously,which was contrary to the inference that IgM might represent the precursor of pathogenic IgG to DNA;4.peak and positive percentage between IgG or IgM against ds-DNA and ss-DNA were different,suggesting that B cells tolerance to ss-DNA in primary B cells repertoire might be more complete than tods-DNA; 5.HSP43 was the key molecule of C jejuni-induced murine model with systemic autoimmune syndromes.Our results supported the hypothesis that immune response to HSP may be highly cross-reactive and even involve autoimmune diseases.