莲雾组织培养和快速繁殖技术

Technique of tissue culture and rapid propagation for wax jumbu

以莲雾未萌发带顶芽茎段为材料研究其组织培养和快速繁殖技术。结果表明:莲雾组织培养的基本培养基为WPM;带顶芽茎段诱导芽的适宜培养基为WPM+0.5 mg.L-16-BA+0.02 mg.L-1NAA;通过腋芽增殖途径的适宜培养基为WPM+0.8 mg.L-16-BA+0.4 mg.L-1IBA;壮苗适宜培养基为WPM+0.2 mg.L-16-BA+0.4 mg.L-1IBA;生根适宜培养基为1/2WPM+0.5 mg.L-1NAA+0.2 mg.L-1IAA。组培苗驯化移栽成活率达90%以上。剪取成活组培苗顶部枝梢进行嫩枝(幼态)扦插生根成苗,能提高繁殖率,降低成本。

A study was conducted on the technique of tissue culture and rapid propagation with the stem segments of wax jumbu as material. The results showed that WPM medium was its basic one, WPM ＋0.5 mg · L^-1 6-BA ＋ 0.02 mg · L^-1 NAA was suitable medium for generation of induction bud from stem segments with terminal bud, WPM ＋0.8 mg · L^-1 6-BA ＋0.4 mg · L^-1 IBA for multiplication of the buds with germination of axillary buds, WPM ＋ 0.2 mg · L ^- 1 6-BA ＋ 0.4 mg · L ^- 1 IBA for sound seedling, 1/2 WPM ＋0.5 mg · L^-1 NAA ＋0.2 mg · L^-1 IAA for rooting of shootings. The rate of survival after habituation was over 90%. The method which is to cut the tip of a branch from tissue cultured seedlings survival in transplant for cuttage, could enhance rate of multiplication and reduce cost.