金花茶成年树茎段离体器官发生

In vitro organogenesis of stem sections from adult trees of Camellia nitidissima Chi.

以金花茶成年树茎段为外植体进行离体器官发生研究。结果表明:分段消毒法是较为理想的消毒方式;附加2 mg.L-1BA及0.5 mg.L-1IAA的改良WPM培养基适合诱导腋芽萌发,萌发率达57.1%;芽苗在附加3 mg.L-1BA及0.2 mg.L-1IAA的改良WPM培养基上增殖效果较好;在附加4 mg.L-1或6 mg.L-1NAA的1/2MS培养基上可诱导芽苗生根。

The system of organogenesis in vitro from the stem segments of adult trees was established in Camellia nitidissima. The results showed that the effective method of surface sterilization for the explants was discontinuous sterilization; the modified solid WPM medium supplemented with 2.0 mg · L^-1 BA and 0.5 mg · L^-1 IAA was suitable for inducing germination of stem auxiliary buds, and the rate of germination was up to 57.1% ; the modified solid WPM medium with 3.0 mg· L^-1 BA and 0.2 mg · L^-1 IAA was suitable for multiplication of shoot; and the multiplication of shoots rooted in the solid 1/2MS medium supplemented with 4 mg · L^-1 or 6 mg·L^-1 NAA.