锂对大鼠海马CCK蛋白表达和学习记忆的影响

Effects of lithium on rat hippocampus cholecystokinin protein expression and learning and memory

目的观察慢性给锂大鼠海马胆囊收缩素(CCK)蛋白表达的变化及对体重和学习记忆能力的影响,阐明锂长期作用对脑发育及学习记忆能力的影响。方法通过测量体重,利用Y迷宫试验和ABC免疫组化法,研究用含不同剂量(3、30、300、3000mgkg)氯化锂(LiCl)饲料喂养的大鼠体格发育情况、学习记忆能力以及海马不同亚区CCK阳性神经元的变化。结果3、30mgkg组大鼠体重增量和Y迷宫实验成绩均明显好于正常对照组(P<0.01),300、3000mgkg组大鼠则明显差于对照组(P<0.05)。3、30、300mgkg组大鼠海马CCK蛋白表达较对照组增强,3000mgkg组则明显减弱(P<0.05)。结论较低浓度的锂能促进大鼠体格发育,提高学习记忆能力,增强海马CCK蛋白表达;而高浓度锂会产生负面影响。

Objective To investigate the effects of lithium on brain development, leaning and memory, we observed the effects of lithium chloride on rat body weight, learning ability and memory capacity, Methods Wistar rats were randomly divided into control group and four lithium chloride （IACI） groups. Four IACI groups were feed with food containing 3,30,300,3000mg/kg LiCl respectively. Control group was feed with normal food. By means of measuring the body weight, using Y-maze test and ABC immunohistochemistry, we observed the difference of the body weight gains and Y-maze training times of different groups, and the changes of CCK positive neurons in hippocampus. Rusults Compared with control group, 3,30mg/kg LiCI groups could increase the body weight and improve the ability of learning and memory of rats（P 〈 0.01）, however, these of the rats of 300,3000mg/kg group were lower（ P 〈 0.05）. The numbers of CCK positive neurons in hippocampus of 3,30,300mg/kg groups were significantly increased compared with control group（ P 〈 0.05）, that of 3000mg/kg group was lower than control group（ P 〈 0.05）. Conclusion Low dose of lithium can improve the growth, the learning ability and the memory capacity obviously, but high dose of lithium could result in harmful effects.