PCR法检测食品和动物饲料中的转基因成分

Detection of genetically modified organisms in food and animal feed by polymerase chain reaction

目的初步调查广州市场上含大豆及玉米原料的食品和饲料中转基因成分的存在情况。方法通过PCR检测camv35S启动子和nos终止子,筛选食品中是否含转基因成分,并进一步通过PCR检测RoundUpReadyTMSoybean(RRS)、Bt176Maximaizer和Mon810YieldGard的特异DNA片段,判断是否含这三种成分。结果从22份市售食品和3份动物饲料,检测出1份玉米汤和2份鲜黄豆样品、1份薯条、及1份大鼠和1份豚鼠饲料中含转基因成分;其中的阳性鲜黄豆和饲料样品含有RRS,而阳性玉米汤和饲料样品中未检测到Bt176Maximaizer和Mon810YieldGard。结论部分未标识市售食品和动物饲料中含转基因成分。

Objective To investigate the presence of genetically modified organisms （GMO） in the foods and animal feed samples in Guangzhou market. Methods The presence of GMO were investigated by PCR detection of camv 35S promoter and nos terminator, and the presence of RoundUp ReadyTM Soybean （RRS）, Bt176 Maximaizer or Mon810 YieldGard in GMO-positive samples were further determined Results One corn soup sample, two soybean samples, one by PCR detecting their specific DNA fragments respectively. potato fries sample as well as two animal feed samples were revealed to be GMO-positive in twenty-two food samples and three animal feed samples, and the presence of RRS in the GMO-positive soybean samples and the two positive animal feed samples were verified by PCR detection of a 129 bp RRS specific DNA fragment, however, no Bt176 Maximaizer or Mon810 YieldGard specific PCR products were obtained with the GMO-positive corn soup and animal feed DNA samples used as PCR templates. Conclusion Genetically modified organism presented in foods and animal feeds even though they were not been labelled.