矿化胶原复合物引导组织再生膜体外性能测试

In vitro fest of mineralized collagen-based composite as guided tissue regeneration membrane

目的:研究一种新型可吸收性引导组织再生(GuidedTissueRegeneration,GTR)膜的机械力学性能,以及其体外降解过程。方法:通过在聚乳酸(Polylactide,PLA)中加入矿化胶原复合物(nano-hydroxyapatite/collagen,nHAC)获得复合GTR膜,矿化胶原中的无机物为纳米相的羟基磷灰石。测量不同nHAC含量的复合膜的弹性模量和最大拉伸强度,通过热失重分析评估模拟体液浸泡后样本无机相的变化,并通过扫描电镜观察样本表面形态的变化。结果:当nHAC/PLA比例为0.4:1,各项力学性能指标达到最高值。复合膜表面可以观察到比较活跃的晶体溶解和再沉积过程。结论:在本实验条件下,复合GTR膜nHAC/PLA比例为0.4:1力学性能最佳。体外情况下复合膜表面有较活跃的晶体溶解和再沉积过程。

Objective： To investigate the biodegradability and mechanic behavior of a novel mineralized nano-hydroxyapatite/collagen/poly lactic acid （nHAC/PLA） composite as GTR membrane in vitro. Methods： Powder of nano-hydroxyapatite/collagen was added into PLA to get the composite GTR membrane. The elastic modulus and maximum tensile strength of GTR film samples with different nHAC/PLA ratio were measured to get an optimal nHAC/PLA ratio. Thermo gravimetric analysis was conducted to evaluate the change of the inorganic component in the samples during the process of in vitro aging. Morphology of samples was checked by using scanning electron microscopy. Results： When the nHAC/PLA ratio of the membrane is 0.4：1, the values of mechanical property of the film reach the peak value. There is active dissolution and deposition process of crystals which is propitious to the bone formation on the surface of the composite membrane. Conclusions： In the condition of this experiment, there is the optimal mechanical property of the film when the nHAC/PLA ratio of the novel membrane is 0.4：1. There is active dissolution and deposition process of crystals on the film in the in vitro condition.