应用多重聚合酶链反应检测结核杆菌

Detection of Mycobacterium Tuberculosis by Multiplex Polymerase Chain Reaction

作者合成两对引物，建立结核杆菌ＤＮＡ多重ＰｃＲ法，用以检测分枝杆菌标准菌株，仅人型和牛型结核杆菌呈阳性，灵敏度≥７ｃｆｕ结核杆菌呈阳性。４０例肺结核患者痰，经多重ＰＣＲ法检测２７例呈阳性，而采用Ｂａｃｔｃｃ４６０ＴＢ系统快速培养法培养仅１９例阳性；多重ＰＣＲ法栓出率较一对引物ＰＣＲ法高１５％～１７．５％，具有较好的敏感性和林异性。

wo pairs of primers were synthesized and a multiplex PCR method in detecting M. tuberculosisDNA was developed. The standard strain of Mycobacterium was detected by the multi-PCR. The resultshowed that only M. tuberculosis and M. Bovis were positive. The detective sensitivity of multi-PCRwas≥7 CFU for M. tuberculosis,The sputa of 40 patients with pulmonary tuberculosis were examinedby this method and by the bactec 460 TB system. The positive results in mulit-PCR were 27/40 and inbactec method 19/40( P <0.05).The detection rate of multi-PCR was 15%～17.5%higher than thatwith one pair of primers( P<0. 025).