摘要
从人胎肝组织纯化肝细胞生长刺激物质（ｈＨＳＳ），经过匀浆、热处理、乙醇沉淀、ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ５０离子交换层析和ＳｅｐｈａｄｅｘＧ７５凝胶过滤，聚丙烯酰胺梯度凝胶电泳及ＩＳＣＯＣｏｎｃｅｎｔｒａｔｏｒ转移浓缩电泳等步骤，分离出具有ＨＳＳ活性的蛋白质。经ＳＤＳ－聚丙烯酰胺凝胶电泳显示５３ＫＤ与１８ＫＤ两条带。
uman Hepatic Stimulator Substance(hHSS)has been purified from human fetal liver,The purifi-cation steps include heating at 95 C for 15min. 40%cold ethanol precipitation,ion-exchange chro-matography on DEAE-Sephadex S50 column and gel filtratiori on Sephadex G 75,Polyacrylamide GelElectrophoresis(PAGE)and ISCO Concentrator transfer Electrophoresis. The protein with hHSS ac-tivity has been obtained.SDS-PAGE showed two bands with Mr of 18 KD and 5 3 KD.
出处
《中国医科大学学报》
CAS
CSCD
1996年第1期12-15,共4页
Journal of China Medical University
基金
国家自然科学基金
关键词
肝细胞
刺激物质
肝细胞生长因子
胎肝纯化
hepatocyte
stimulator Substance
hepatocyte growth factor
purification of fetal liver
