摘要
用南瓜的愈伤组织作受体进行了基因枪的轰击试验,确立了以轰击压力为1 100 psi,轰击距离为9 cm,3μLDNA／mg金粉的轰击比例,优化了基因枪的轰击参数;在轰击前4 h和轰击后16 h用附加有0．4 mol／L甘露醇的MS培养基进行高渗处理、轰击后恢复培养6 d,可以增加转化成功的机率。用1 mg／LBasta作 为选择压,筛选培养45 d后进行GUS基因组织化学染色,结果表明GUS基因可在南瓜的组织中瞬时表达。
Pumpkin callus as accptor were transformed via gene-gun bombarment. Bombarment parameters were optimised as： the pressure is 1 100 psi, the distance is 9 cm, 3 μL DNA/mg golden particles. MS medium added 0.4 mol/L mannit was used to do hypertonic treatment 4hours before bombarment and 16 hours after bombarment. This can increase the successful probability of transformation. GUS gene was stained after selected culturing 45 days. The results showed that GUS gene has expressed for a time in pumpkin tissues.
出处
《西北农业学报》
CAS
CSCD
北大核心
2005年第4期97-99,共3页
Acta Agriculturae Boreali-occidentalis Sinica
基金
河南省科技厅计划项目-南瓜新品种选育与产业化研究(编号0424070050)的部分内容。
关键词
南瓜
基因枪
遗传转化
瞬时表达
Pumpkin
Gene gun
Genetic transformation
Expressed for a time
