人精液中NO和ACE、HYD、ACP的检测

Detection of nitric oxide and acrosome enzyme,hyaluronidase,acid phosphatase in human semen

目的:探讨男性不育精液中一氧化氮(NO)和顶体酶(ACE)、透明质酸酶(HYD)、酸性磷酸酶(ACP)的关系。方法:参照WHO标准方法,进行精液常规分析,按精子密度、活动率不同分为4组。采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO3-)。用改良的Kennedy法检测精子ACE活性。用S inger法测HYD活性。采用磷酸苯二钠法检测ACP。结果:72例不育各组NO含量明显高于,而ACE、HYD、ACP活性则明显低于已育组(P<0.01),随精子密度、活动率减少和畸形率增高而NO含量升高,ACE、HYD、ACP活性降低。结论:精液酶检测是评价精子受精能力的重要指标,NO对精子酶活性有抑制灭活影响精子的受精能力。

Objective：To study the relationship between nitric oxide （NO） and the activity of acrosome enzyme （ACE）, hyaluronidase （HYD）, acid phosphatase （ACP） in infertile man semen . Methods： According to the density and activity of the sperms, they were divided into 4 groups, and their routine analysis were referred to the WHO standard method. A coppered reduced cadmium fluometric assay was used to detect nitrate, a metabolic product of nitric oxide in sperm . The activity of ACE was measured by improved kennedy assay, HYD activity was detected by singer assay, and ACP activity was examined by di - Sodium phenyl phosphate. Results： In the different groups of 72 infertile men, compared to the control, NO was significantly higher, but the activity of ACE, HYD, ACP was markedly lower. With the decrease of density, mobility and the increase of deformity of sperm, NO got higher and higher, but the activity of ACE, HYD, ACP became lower and lower. Conclusion： The detection of special enzymes in human semen is an effective index to appraise the fertilization ability of sperm. NO can inhibit the activity of the semen enzymes and may affect the fertilization ability of semen.