正交试验法优选丹参的提取新工艺

Optimum Extraction of Salvia miltiorrhiza Bge. by Orthogonal Test

目的:研究丹参的提取新工艺。方法:以丹参酮ⅡA和丹酚酸B的含量为指标,采用正交试验法进行丹参提取工艺全面优选,并用HPLC法测定其含量。结果:醇提与水提方法相结合,可同时对丹参的脂溶性及水溶性有效成分进行全面提取。在本试验条件下,其中丹参醇提最佳工艺为:丹参加6倍量90%乙醇,回流提取两次,每次1h;丹参水提最佳工艺为:丹参醇提后药渣加7倍量水煎煮两次,每次1h。结论:丹参提取新工艺可兼顾丹参的脂溶性和水溶性活性成分,使丹参的提取工艺合理可行。

Objective：To study the new extract process of Salvia miltiorrhiza Bge.. Methods： Salvianolic acid B and tanshinone ⅡA were selected as marker component and determined by HPLC to optimize the extract process of Salvia miltiorrhiza Bge. by orthogonal test. Results： the aquatic and hydrophobic active components of Salvia miltiorrhiza Bge. were efficiently extracted by the combination of alcohol extraction and water extraction. The optimum alcohol extraction was： Salvia miltiorrhiza Bge. was added with 6 times alcohol of 90% and extracted 1 hour for 2 times; the optimum water extraction was： after the alcohol extraction, the Salvia miltiorrhiza Bge. was added with 7 times water and extracted 1 hour for 2 times. Conclusion： the aquatic and hydrophobic active components of Salvia miltiorrhiza Bge. can be extracted simultaneously by the novel extraction process which was reasonable and feasible.